Paddy is economically important crop that produce rice, ensure food security of billions of people around the world as staple food, and provide livelihoods in rural areas. In the present study, survey the several villages and blocks of Ghazipur district and collected 96 seed samples of paddy and brought to laboratory, and stored at room temperature (20-28°C) for six months. Twenty fungal species were isolated from both external and internal surface by single spore technique. Among them five fungal sp. viz. Aspergillus flavus, A. niger, Curvularia lunata, Fusarium moniliformae and Penicillium sp. were associated both externally and internally on paddy seeds.