2025, Vol. 5, Issue 1, Part A
Isolation and identification of endophytic fungi isolated from Cupressus sempervirens and study of their antimicrobial activity
Author(s): Ban Mousa Hassan
Abstract: Through the study, some fungi grown on Cupressus sempervirens leaves were isolated and identified, and their antifungal activity was studied by testing their extracts against some plant pathogenic fungi and some dermophytic fungi. The tests were conducted at the University of Karbala - College of Education for Pure Sciences - Life Sciences Department Laboratories for the period from 6/1/2023 to 12/1/2023. Through the study, we obtained six genera of fungi cultured on Cupressus sempervirens leaves by growing them on PDA media, namely
Aspergillus flavus, Aspergillus niger, Penicillium sp.,
Fusarium sp.,
Alternaria alternata and
Cladosporium tenellium. Also, the activity of antagonism was tested between the isolated cultured fungi and three types of dermatophytes Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis using Dual culture on SDA medium. The susceptibility of
S. aureus,
P. auroginosa and
E. coli to the isolated fungi was also tested on Mueller Hinton Agar (MHA) media. The results of this study indicate a significant difference in the effectiveness of the antagonism against these pathogens, according to the cultured fungus and the pathogen. The antimicrobial efficacy was evaluated by inhibition ratios, and this result indicates the efficiency of using cultivated fungi in agricultural applications as biological control agents against plant fungal pathogens as well as a good source of natural antimicrobial agents.
DOI: 10.22271/27893065.2025.v5.i1a.111Pages: 18-23 | Views: 97 | Downloads: 39Download Full Article: Click Here
How to cite this article:
Ban Mousa Hassan.
Isolation and identification of endophytic fungi isolated from Cupressus sempervirens and study of their antimicrobial activity. Int J Plant Pathol Microbiol 2025;5(1):18-23. DOI:
10.22271/27893065.2025.v5.i1a.111