Author(s): Bamidele Helen Olaide, Fawole Oluyemisi Bolajoko and Balogun Olusegun Samuel

Abstract: This study was to carry out the molecular characterization of isolated bacterial from some deteriorated fruits and vegetables. Common bacterial affecting selected fruits and vegetables were isolated. They include: Pseudomonas aeruginosa, Salmonella enterica, Enterococcus faecium and Lactobacillus plantarum. They were molecularly characterized using 16SrDNA sequence analyses. DNA extraction was carried out using Zymo DNA mini kit prep and the primer used were 16SF and 16SR. The 16SrDNA regions of these pathogens were amplified by polymerase chain reaction (PCR) with Gene Amp 9700 thermocycler and then sequenced using Applied Biosystem 3130X gene analyser. Nucleotide sequences were deciphered using nBLAST. Alignment and construction of phylogenetic tree were performed using Neighbour Joining method of MEGA 7 software together with the sequence of related strains which were downloaded from GenBank. The results of the DNA yield and purity were within the range of 23 to 215.2 ng/μl and 1.84 to 1.93. The number of nucleotide for all the organisms isolated varies from 490 to 1563, BLAST searches revealed that the organisms identified were within the range of 93-100% identical. The results demonstrated that good yield and pure DNA extracts were obtained from the selected fruits and vegetables and the primers used are useful for bacterial identification.

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